Objective To explore the effect of hirudin on the expression of matrix metalloproteinases of the human hypertrophic scar fibroblast.Methods 10 cases of hypertrophic scar tissue were taken from the operated patients who were within 6 months after injury.Fibroblasts of hypertrophic scar tissue were cultured by tissue block method.The cells after 4?7generations were used in experiments, which were interfered with exposure to 0U/ml, 1U/ml, 10U/ml or 50U/ml hirudin for 24 and 48 hours.We detected the effect of hirudin on the proliferation of fibroblasts by MTT method and the expression of MMP-2, MMP-9 by enzyme-linked immunosorbent assay (ELISA).Results ?Hirudin could inhibit the proliferation of scar fibroblasts in the group of 1, 10, 50U/ml (P0.05).The effect of inhibition of hirudin with 50U/ml was most significant (the inhibition rates of 24, 48hours were 14.75% and 15.42%).?The level of expression of MMP-2 and MMP-9 was increased with the role of hirudin on fibroblasts after 24h, especially it was most significant with 1U/ml and 50U/ml.The difference was statistically significant (P0.05).After 48hours the expression of MMP-2 was decreased, meanwhile the expression of MMP-9 was increased but no significance.Conclusion Hirudin can inhibit the proliferation of hypertrophic scar fibroblasts, promote the secretion of matrix metalloproteinases (MMP-2, MMP-9) by hypertrophic scar fibroblasts and will help to promote the degradation and reduction of the extracellular matrix.
--LI Kai-tong, LIU Da-en, LI Shun-tang, LU Hai-qiang, CHEN Xiao-ting, GAO Xing-xin. Effect of Hirudin on Matrix Metalloproteinases of Human Hypertrophic Scar Fibroblast. Zhong Guo Mei Rong Yi Xue. 2012; 21 (2): 247-250.