Objective: To explore the effects of Opuntia on fibroblast proliferation in vitro and its concentration-effect relationship. Methods: The effects of Opuntia on proliferation of fibroblast were measured with MTT colorimetric assay, and PCNA productions were examined with immunocytechemistry. Results: Opuntia could provoke fibroblast proliferation and increase PCNA protein expression in concen- tration range 0.0625?0.5 mg/mL. Furthermore, the effects exhibited a significant concentration-dependent relationship (r=0.761, 0.783 respectively, P<0.01). Conclusion: Opuntia can provoke fibroblast prolif- eration within a certain concentration range, which exhibits a significant concentration-effect relationship.
--JIN Zhao; JIN Ke-ke; CHEN Lei; LIU Chang-bao. Effects of Opuntia on fibroblast proliferation in vitroWen Zhou Yi Xue Yuan Xue Bao. 2009; 39 (5): 436-438.