Objective To investigate the protective effects of astragaloside on the oxidative damage induced by ultraviolet-A (UVA) irradiation and its possible mechanisms. Methods Subconfluent fibroblasts were shammed or irradiated with different dosages of UVA irradiation and treated with astragaloside. Cellular activity was detected by MTT assay. The activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) were detected by colorimetric methods. Results The proliferation vality of fibroblasts descended with the U VA irradiation (5, 10J/cm^2), and the activities of SOD decreased, the content of MDA was ascensive (P<0.05). But under the intervention of astragaloside, the above changes induced by UVA irradiation were inhibited (P<0.05). Conclusion Astragaloside might inhibit the proliferation decreasing which induced by UVA. And its mechanism may have relationship with the ability of strengthening antioxidation and decreasing oxygen radicals.
--WANG Mei-ling, WU Xiu-hong, ZHOU Jing-fen, WANG Rong, WANG Bao-tao, YA NG Zi-liang. Effects of Astragaloside on Oxidative Damage of Human Fibroblasts Induced by Ultraviolet-A Irradiation in Vitro. Zhong Guo Zhong Xi Yi Jie He Pi Fu Xing Bing Xue Za Zhi. 2009; 8 (5): 268-270.