[Objective] To investigate the effects of matrine on the proliferation of lung fibroblasts and expression of fibronectin (FN) in rat. [Methods] Cultured lung fibmblasts of neonatal Wistar rats were treated with alatrine. Lung fibroblasts prolifertion was measured by thlazolyl blue (MTT) assay; cell cyclin distribution was determined with flow cytometre; immunofluorescence staining detected the expression of FN of lung fibroblasts. [Results] After treating with Matrine, the value of lung fibroblasts decreased in concentration-dependent manner, concomitant with a significant increase in the percentage of G0/G1 stage cells. FN expression in lung fibroblasts was downregulated by matrine. [ Conclusion ] Matrine inhibits effectively the pmlifertion of cultured lung fibroblasts in rat by downregulating FN expression.
--ZHOU Yan-fang, HUANG Pei-chun, OUYANG Jing-ping. Effect of matrine on proliferation of lung fibroblasts in rat. Zhong Guo Xian Dai Yi Xue Za Zhi. 2006; 16 (8) : 1181-1183, 1187.
There is no satisfactory treatment for pulmonary fibrosis, which is characterized by altered control of proliferation of mesenchymal fibroblasts and extracellular matrix production. Oxymatrine is an alkaloid extracted from the Chinese herb Sophora japonica (Sophora flavescens Ait.) with capacities of anti-inflammation, inhibition of immune reaction, antivirus, protection against acute lung injury and antihepatic fibrosis. In this study, the effect of oxymatrine on pulmonary fibrosis was investigated using a bleomycin-induced pulmonary fibrosis mouse model. The results showed that bleomycin challenge provoked severe pulmonary fibrosis with marked increase in hydroxyproline content of lung tissue and lung fibrosis fraction, which was prevented by oxymatrine in a dose-dependent manner. In addition, bleomycin injection resulted in a marked increase of myeloperoxidase activity and malondialdehyde level that was attenuated by oxymatrine. Administration of oxymatrine inhibited the proliferation of murine lung fibroblasts, arrested the cells at G (0)/G (1) phase and reduced the expression of cell cycle regulatory protein, cyclin D1 in vitro. Furthermore, the steady-state production of collagen and the expression of alpha1 (I) pro-collagen and alpha2 (I) pro-collagen mRNA in fibroblasts were inhibited by oxymatrine in a dose-dependent manner. These results suggested that oxymatrine may attenuate pulmonary fibrosis induced by bleomycin in mice, partly through inhibition of inflammatory response and lipid peroxidation in lung induced by bleomycin and reduction of fibroblast proliferation and collagen synthesis.
--Chen, X., R. Sun, J. Hu, Z. Mo, Z. Yang, D. Liao, N. Zhong. Attenuation of bleomycin-induced lung fibrosis by oxymatrine is associated with regulation of fibroblast proliferation and collagen production in primary culture. Basic Clin Pharmacol Toxicol, 2008. 103 (3):278-286.